林翠华

（泰安市食品药品检验检测研究院（泰安市纤维检验所），泰安271000）

摘要：建立超高效液相色谱-串联质谱法测定粽子专项抽检样品中安赛蜜含量的分析方法。采用Agilent SB-C18色谱柱（2.1mm×100mm，1.8μm）；以0.1%甲酸水溶液-乙腈为流动相，梯度洗脱；流速0.2mL/min，柱温35℃。结果表明，安赛蜜在10～200 ng/mL浓度范围内线性关系良好，相关系数（R2）为0.999 5，加标回收率平均为99.5%，相对标准偏差（RSD）为0.81%。通过超高效液相色谱-串联质谱法测定15批粽子中的安赛蜜含量，发现2批粽子检测出安赛蜜，检出率为13.3%，安赛蜜的含量分别为0.057g/kg、0.076g/kg。该方法具有前处理简单、准确度高、检测速度快等优点。

关键词：粽子；安赛蜜；超高效液相色谱-串联质谱法；含量测定

中图分类号：TS213.23 文献标识码：A 文章编号：1674-506X（2025）01-0164-0005

Determination of Acesulfame Content Added in Rice Dumplings by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LIN Cuihua

（Tai’an Food and Drug Inspection and Testing Institute（Tai’an Fiber Inspection Institute），Taian 271000，China）

Abstract：To establish an analytical method for the determination of acesulfame content in rice dumplings particular spot-check samples by ultra performance liquid chromatography-tandem mass spectrometry. The chromatographic column used in this method was Agilent SB-C18 column (2.1 mm×100 mm, 1.8 μm); 0.1% formic acid aqueous solution-acetonitrile was used as mobile phase for gradient elution; The flow rate was 0.2 mL/min, and the column temperature was 35 ℃. The results showed that acesulfame had a good linear relationship in the range of 10～200 ng/mL, the correlation coefficient (R2) was 0.999 5, the average spiked recovery rate was 99.5% , and the relative standard deviation (RSD) was 0.81% . Acesulfame content was determined in 15 batches of rice dumplings by ultra performance liquid chromatography- tandem mass spectrometry, acesulfame was detected in 2 batches of rice dumplings, the detection rate was 13.3%, the contents of acesulfame were 0.057 g/kg and 0.076 g/kg respectively. The method has the advantages of simple pretreatment, high accuracy and fast detection speed.

Keywords：rice dumplings； acesulfame； ultra performance liquid chromatography- tandem mass spectrometry；content determination

doi：10.3969/j.issn.1674-506X.2025.01-022